Skip to main content
Figure 3 | Microbial Cell Factories

Figure 3

From: In vivo production of a novel glycoconjugate vaccine against Shigella flexneri 2a in recombinant Escherichia coli: identification of stimulating factors for in vivo glycosylation

Figure 3

Impact of order of induction on EPA-2a yield. For EPA-2a production engineered E. coli were incubated at 30°C in shake flasks, and PglB and EPA carrier protein were induced either simultaneously (0 h) with 1 mM IPTG and 2 g/L arabinose, respectively, or EPA was induced at indicated time points after PglB induction. OD600-normalized periplasmic extracts were prepared 24 h post induction and analyzed by sandwich ELISA. Error bars represent the standard deviations of three biological replicates.

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us