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Figure 1 | Microbial Cell Factories

Figure 1

From: In vivo production of a novel glycoconjugate vaccine against Shigella flexneri 2a in recombinant Escherichia coli: identification of stimulating factors for in vivo glycosylation

Figure 1

In vivo glycosylation of EPA with Shigella flexneri 2a O-polysaccharides. E. coli expressing Shigella flexneri 2a polysaccharides, EPA carrier protein and either PglB or PglBmut were grown in shake flasks at 30°C and induced with 1 mM IPTG and 2 g/L arabinose or retained uninduced. 24 h post induction OD600-normalized periplasmic extracts were prepared and analyzed by Western blot with A) anti-EPA antibody or B) anti-2a antibody (B: right panel overexposed Western blot). C) Periplasmic extracts from A) and B) were analyzed and quantified by sandwich enzyme-linked immunosorbent assay (ELISA). Error bars represent the standard deviations of three biological replicates.

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Microbial Cell Factories

ISSN: 1475-2859

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