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Table 2 Transfer efficiencies of pWD2-oriT between E. coli and Pseudoalteromonas strains

From: Development of an efficient conjugation-based genetic manipulation system for Pseudoalteromonas

Recipient strains

CFU*

Transconjugants &

Efficiency

A37-1-2

4.7 × 107

3.0 × 104

6.4 × 10−4

TAC125

2.5 × 107

1.8 × 102

7.2 × 10−6

SM20429

3.2 × 107

1.4 × 104

4.4 × 10−4

SM9913

3.8 × 107

1.6 × 104

4.2 × 10−4

DSM 16099

1.5 × 108

1.6 × 103

1.1 × 10−5

DSM 16098

9.5 × 107

1.3 × 102

1.4 × 10−6

DSM 6842

8.7 × 107

9.4 × 101

1.1 × 10−6

SCSIO 04301

9.7 × 107

1.6 × 105

1.6 × 10−3

SCSIO 11900

3.0 × 107

9.8 × 101

3.3 × 10−6

  1. *Average number of recipient cells in two replicates.
  2. &Average number of transconjugants in two replicate conjugations plated in triplicate plates; approximately 1/10th of the total conjugation volume was plated.
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Microbial Cell Factories

ISSN: 1475-2859

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