Figure 7
From: Development of an efficient conjugation-based genetic manipulation system for Pseudoalteromonas
Confirmation of in-frame deletion of flagellar motor protein genes in SCSIO 11900. (A) PCR detection of fliFG single crossover mutants using two primer pairs. M, DNA Marker III. PCR templates used are: 1 and 2, two independent colonies that can grow on the selective plate after mating. WT indicates wild-type SCSIO 11900. NC indicates negative control ddH2O. (B) PCR confirmation of the mutants that underwent a second homologous recombination using the fliFG-wS and fliFG-wA primers. 1, one colony after the second homologous recombination. WT indicates wild-type SCSIO 11900. NC indicates negative control ddH2O. (C) Swimming motility of wild-type SCSIO 11900 and ΔfliFG.