Figure 6
From: Development of an efficient conjugation-based genetic manipulation system for Pseudoalteromonas
Confirmation of in-frame deletion of hmgA in SCSIO 04301. (A) PCR detection of hmgA single crossover mutants using two primer pairs. M, DNA Marker III. PCR templates used are: 1, one colony that can grow on the selective plate after mating. WT indicates SCSIO 04301 wild-type strain. NC indicates negative control ddH2O. (B) PCR confirmation of the mutants that underwent a second homologous recombination using the hmgA-wS and hmgA-wA primers. 1, one colony after the second homologous recombination. WT indicates SCSIO 04301 wild type. NC indicates negative control ddH2O. (C) The pigment production of wild-type SCSIO 04301, ΔhmgA mutant, ΔhmgA/pWD2-oriT and ΔhmgA/pWD2-oriT-hmgA.