Figure 5

Confirmation of in-frame deletion of bsmA in SM9913. (A) PCR detection of bsmA single crossover mutants using three primer pairs. M, DNA Marker III. PCR templates used are: 1–4, four independent colonies that can grow on the selective plate after mating. WT indicates SM9913 wild-type strain. NC indicates negative control ddH₂O. (B) PCR confirmation of the mutants that underwent a second homologous recombination using the bsmA-wS and bsmA-wA primers. 1–2, two independent colonies after the second homologous recombination. WT indicates SM9913 wild type. NC indicates negative control ddH₂O. (C) Biofilm formation of wild type SM9913 and ΔbsmA strains. Normalized biofilm formation (total biofilm/growth) in SWLB medium at 20°C after 1 d, 2 d, 3 d, and 4 d in 96-well plates. Data are the average of ten replicate wells from two independent cultures, and one standard deviation is shown. (D) Swimming motility of wild type SM9913 and ΔbsmA.