Figure 4
From: Development of an efficient conjugation-based genetic manipulation system for Pseudoalteromonas
Confirmation of in-frame deletion of prodigiosin biosynthesis genes in DSM 6842. (A) PCR detection of pigM-K single crossover mutants using two primer pairs. M, DNA Marker III. 1–6, six independent colonies that can grow on the selective plate after conjugation. WT, DSM 6842 wild-type strain. NC, negative control ddH2O. (B) PCR confirmation of the mutants that underwent a second homologous recombination using the pigM-wS and pigM-wA primers. 1–3, three independent colonies after the second homologous recombination. WT, wild-type strain DSM 6842. NC, negative control ddH2O. (C) The prodigiosin production of three ΔpigM-K mutants and the wild-type strain DSM 6842.