Figure 3
From: Development of an efficient conjugation-based genetic manipulation system for Pseudoalteromonas
Schematics illustrating the use of the pK18 mobsacB -Cm or pK18 mobsacB -Ery suicide plasmids to generate a defined, marker-free deletion in Pseudoalteromonas strains. (A) Construction of suicide plasmid constructs containing the homologous fragments flanking the target DNA region. (B) First recombination event by integration into the chromosome via homologous recombination and position of the primers for plasmid insertion verification. (C) The second recombination event by sucrose selection, and position of primers to separate the deletion mutants from the wild-type.