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Figure 8 | Microbial Cell Factories

Figure 8

From: High yield purification of full-length functional hERG K+ channels produced in Saccharomyces cerevisiae

Figure 8

Detergent screen of crude membranes from PAP1500 producing hERG-TEV-GFP-His 8 at 15°C. Membrane proteins were solubilized as described in the Methods section, at the indicated detergent/protein ratios and a cholesteryl-hemisuccinate concentration of 4.25; 2.8 or 2 mg/ml for the 3 ratios, respectively. Abbreviations; FC-12, Fos-Choline-12; LDAO lauryldimethylamine N-oxide; CYMAL5, 5-Cyclohexyl-1-pentyl- β-D-maltoside ; DDM, n-Dodecyl- β-D-maltopyranoside; DM, n-Decyl- β-D-maltopyranoside; C 12 E 8, Octaethylene glycol monododecyl ether; CHAPS, 3-[(3-cholamidopropyl) dimethylam-monio] -1-propanesulfonate; OG, n-Octyl- β-D-glucopyranoside. Solubilization was determined as GFP fluorescence of solubilized protein normalized to GFP fluorescence in the crude membranes used for solubilization.

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