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Figure 6 | Microbial Cell Factories

Figure 6

From: High yield purification of full-length functional hERG K+ channels produced in Saccharomyces cerevisiae

Figure 6

Endo-H treatment of crude yeast membranes under non-denaturing conditions. Crude yeast membranes (80 μg) were treated with Endo-H as described in methods in a non denaturing buffer at 4°C over night. Untreated crude membranes (lane 1) or Endo-H treated membranes (lane 2) were separated by SDS-PAGE and analyzed by in-gel fluorescence. The only bands visible are the hERG-TEV-GFP-His 8.

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