Figure 2

Structural map of the hERG-TEV-GFP-His ₈ and hERG-His ₁₀ expression plasmids. Abbreviations used: CG-P, a hybrid promoter carrying the GAL10 upstream activating sequence fused to the 5 ^′ non-translated leader of the cytochrome-1 gene; T, Tobacco Etch Virus (TEV) cleavage site; GFP-His ₈, yeast enhanced GFP cDNA fused to eight histidine codons; 2 μ, the yeast 2 micron origin of replication; leu2-d, a poorly expressed allele of the β-isopropylmalate dehydrogenase gene; bla, a β-lactamase gene; pMB1, the pMB1 origin of replication; URA3, the yeast orotidine-5 ^′-phosphate decarboxylase gene. Rapid construction of expression plasmids was carried out by in vivo homologous recombination in S. cerevisiae.