Figure 12

TEV digestion of hERG-TEV-GFP-His ₈ . Peak fraction 17 from the Ni-affinity purification in Figure 10 was dialyzed for 8 hours at 4°C and subsequently digested over night at room temperature at a hERG-TEV-GFP-His ₈: TEV ratio of 1:10 (w/w). A) In-gel fluorescence of undigested hERG-TEV-GFP-His ₈ protein (lane 1); TEV digested hERG-TEV-GFP-His ₈ protein (lane2). B) Coomassie stain of the gel in A. The 156 kDa band represents the hERG-TEV-GFP-His ₈ fusion, the 127 kDa band is the tag-free hERG, the 36 kDa band is the GFP-tag liberated after TEV digestion and the 25 kDa band is TEV protease.