Figure 10

Purification of hERG-TEV-GFP-His ₈ by Ni-affinity chromatography. HERG-TEV-GFP-His ₈ was solubilized in FC-12/CHS and incubated with Ni-resin over night at 4°C, as described in the Methods section. The Ni-resin was poured on a column and hERG-TEV-GFP-His ₈ was eluted from the Ni-resin using the indicated imidazole step gradient (blue). Fluorescence was measured in each fraction to estimate the elution profile of the hERG-fusion (pink). B) In-gel fluorescence of Ni-affinity purified hERG-TEV-GFP-His ₈ separated by SDS-PAGE in an 8% gel. Lanes contain fractions 13-21 from A. C) Coomassie stain of the SDS-PAGE gel in B.