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Table 1 Strains and plasmids used in this study
| Strains or plasmids | Characteristics | Source |
|---|---|---|
| Strains | ||
| B1 | E. coli BL21(DE3) wild type | Laboratory stock |
| B2 | E. coli BL21(DE3) ΔtrpR | This study |
| B3 | E. coli BL21(DE3) ΔtrpR ΔpheA | This study |
| B4 | E. coli BL21(DE3) ΔtnaA | This study |
| B5 | E. coli BL21(DE3) ΔtnaA ΔpheA | This study |
| B6 | E. coli BL21(DE3) ΔtrpR ΔtnaA | This study |
| B7 | E. coli BL21(DE3) ΔpheA | This study |
| B8 | E. coli BL21(DE3) ΔtrpR ΔtnaA ΔpheA | This study |
| plasmids | ||
| pACYCDuet | Cm | Novagen |
| pRSFDuet | Km | Novagen |
| pED | pACYCDuet with trpE fbr (Met293Thr, TCC to TTC; Ser40Leu ATG to ACG) and trpD genes in NcoI and KpnI sites | This study |
| pVio | pRSFduet with vio gene cluster in NdeI and XhoI sites | This study |
