|
Strains
| | |
|
B1
|
E. coli BL21(DE3) wild type
|
Laboratory stock
|
|
B2
|
E. coli BL21(DE3) ΔtrpR
|
This study
|
|
B3
|
E. coli BL21(DE3) ΔtrpR ΔpheA
|
This study
|
|
B4
|
E. coli BL21(DE3) ΔtnaA
|
This study
|
|
B5
|
E. coli BL21(DE3) ΔtnaA ΔpheA
|
This study
|
|
B6
|
E. coli BL21(DE3) ΔtrpR ΔtnaA
|
This study
|
|
B7
|
E. coli BL21(DE3) ΔpheA
|
This study
|
|
B8
|
E. coli BL21(DE3) ΔtrpR ΔtnaA ΔpheA
|
This study
|
|
plasmids
| | |
|
pACYCDuet
|
Cm
|
Novagen
|
|
pRSFDuet
|
Km
|
Novagen
|
|
pED
|
pACYCDuet with trpE
fbr (Met293Thr, TCC to TTC; Ser40Leu ATG to ACG) and trpD genes in NcoI and KpnI sites
|
This study
|
|
pVio
|
pRSFduet with vio gene cluster in NdeI and XhoI sites
|
This study
|
