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Table 1 Strains and plasmids used in this study

From: High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway

Strains or plasmids Characteristics Source
B1 E. coli BL21(DE3) wild type Laboratory stock
B2 E. coli BL21(DE3) ΔtrpR This study
B3 E. coli BL21(DE3) ΔtrpR ΔpheA This study
B4 E. coli BL21(DE3) ΔtnaA This study
B5 E. coli BL21(DE3) ΔtnaA ΔpheA This study
B6 E. coli BL21(DE3) ΔtrpR ΔtnaA This study
B7 E. coli BL21(DE3) ΔpheA This study
B8 E. coli BL21(DE3) ΔtrpR ΔtnaA ΔpheA This study
pACYCDuet Cm Novagen
pRSFDuet Km Novagen
pED pACYCDuet with trpE fbr (Met293Thr, TCC to TTC; Ser40Leu ATG to ACG) and trpD genes in NcoI and KpnI sites This study
pVio pRSFduet with vio gene cluster in NdeI and XhoI sites This study