Strains or plasmids | Characteristics | Source |
---|---|---|
Strains | Â | Â |
  B1 | E. coli BL21(DE3) wild type | Laboratory stock |
  B2 | E. coli BL21(DE3) ΔtrpR | This study |
  B3 | E. coli BL21(DE3) ΔtrpR ΔpheA | This study |
  B4 | E. coli BL21(DE3) ΔtnaA | This study |
  B5 | E. coli BL21(DE3) ΔtnaA ΔpheA | This study |
  B6 | E. coli BL21(DE3) ΔtrpR ΔtnaA | This study |
  B7 | E. coli BL21(DE3) ΔpheA | This study |
  B8 | E. coli BL21(DE3) ΔtrpR ΔtnaA ΔpheA | This study |
plasmids | Â | Â |
  pACYCDuet | Cm | Novagen |
  pRSFDuet | Km | Novagen |
  pED | pACYCDuet with trpE fbr (Met293Thr, TCC to TTC; Ser40Leu ATG to ACG) and trpD genes in NcoI and KpnI sites | This study |
  pVio | pRSFduet with vio gene cluster in NdeI and XhoI sites | This study |