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Table 1 Strains and plasmids used in this study

From: High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway

Strains or plasmids

Characteristics

Source

Strains

  

  B1

E. coli BL21(DE3) wild type

Laboratory stock

  B2

E. coli BL21(DE3) ΔtrpR

This study

  B3

E. coli BL21(DE3) ΔtrpR ΔpheA

This study

  B4

E. coli BL21(DE3) ΔtnaA

This study

  B5

E. coli BL21(DE3) ΔtnaA ΔpheA

This study

  B6

E. coli BL21(DE3) ΔtrpR ΔtnaA

This study

  B7

E. coli BL21(DE3) ΔpheA

This study

  B8

E. coli BL21(DE3) ΔtrpR ΔtnaA ΔpheA

This study

plasmids

  

  pACYCDuet

Cm

Novagen

  pRSFDuet

Km

Novagen

  pED

pACYCDuet with trpE fbr (Met293Thr, TCC to TTC; Ser40Leu ATG to ACG) and trpD genes in NcoI and KpnI sites

This study

  pVio

pRSFduet with vio gene cluster in NdeI and XhoI sites

This study