Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli

Table 4 Sequences of primers for qPCR

Target	Primers (5′ → 3′) ^a	Length (nt)	Primer position	Product size (bp)
*Bla*	F: AGTTCTGTCTCGGCGCGTCT	20	1331-1350	96
	R: ACTCCAGTCGCCTTCCCGTT	20	1426-1445
*T7 RNApol*	F: AGGACTGCTTACGCTGGCGA	20	1488-1507	116
	R: TGATGCGCTCAGGGAACGGA	20	1603-1622

ISSN: 1475-2859