|
Type A clone
|
Arithmetic mean X/Y ratio
|
Standard deviation [%]
|
Type B clone
|
Arithmetic mean X/Y ratio
|
Standard deviation [%]
|
|---|
|
His-LOV
|
1.7
|
7.1
|
I12C
|
0.6
|
0.5
|
|
Wild type enzyme (His-LOV-BSLA)
|
2.2
|
4.6
|
F17P
|
0.6
|
3.6
|
|
A1W
|
2.2
|
7.9
|
G93Y
|
0.5
|
6.7
|
|
H10D
|
1.4
|
2.0
|
V99K
|
0.5
|
0.4
|
|
K23stop
|
1.5
|
2.9
|
L102W
|
0.4
|
4.5
|
|
S56P
|
1.9
|
3.8
|
K170E
|
0.4
|
3.3
|
|
D91R
|
1.4
|
2.5
| | | |
|
S167P
|
2.4
|
7.5
| | | |
|
G175F
|
1.4
|
1.8
| | | |
|
Type A
|
1.4 – 2.4
| |
Type B
|
0.4 – 0.6
| |
|
Type A*
|
>1.2
| |
Type B*
|
<1.2
| |
- Ratio between integral X (representing protein production phase) and integral Y (representing second growth phase) determined for the 15 investigated E. coli BL21(DE3) clones according to Figure 4. For each clone, the X/Y ratio was calculated for all individual cultivations presented in Figure 3. The arithmetic mean as well as the standard deviation (in %) are presented.
- *Critical X/Y ratio for simplified classification between Type A and Type B clones.
