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Table 2 X/Y ratio of Type A and Type B clones

From: Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli

Type A clone Arithmetic mean X/Y ratio Standard deviation [%] Type B clone Arithmetic mean X/Y ratio Standard deviation [%]
His-LOV 1.7 7.1 I12C 0.6 0.5
Wild type enzyme (His-LOV-BSLA) 2.2 4.6 F17P 0.6 3.6
A1W 2.2 7.9 G93Y 0.5 6.7
H10D 1.4 2.0 V99K 0.5 0.4
K23stop 1.5 2.9 L102W 0.4 4.5
S56P 1.9 3.8 K170E 0.4 3.3
D91R 1.4 2.5    
S167P 2.4 7.5    
G175F 1.4 1.8    
Type A 1.4 – 2.4   Type B 0.4 – 0.6  
Type A* >1.2   Type B* <1.2  
  1. Ratio between integral X (representing protein production phase) and integral Y (representing second growth phase) determined for the 15 investigated E. coli BL21(DE3) clones according to Figure 4. For each clone, the X/Y ratio was calculated for all individual cultivations presented in Figure 3. The arithmetic mean as well as the standard deviation (in %) are presented.
  2. *Critical X/Y ratio for simplified classification between Type A and Type B clones.