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Table 1 Applied clones and abbreviations

From: Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli

Clone Abbreviation
E. coli BL21(DE3):pET22b(+)–His6-LOV His-LOV
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-wt Wild type enzyme (His-LOV-BSLA)
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Ala1Trp A1W
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-His10Asp H10D
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Ile12Cys I12C
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Phe17Pro F17P
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Lys23stop K23stop
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Ser56Pro S56P
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Asp91Arg D91R
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Gly93Tyr G93Y
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Val99Lys V99K
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Leu102Trp L102W
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Ser167Pro S167P
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Lys170Glu K170E
E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Gly175Phe G175F
  1. The investigated E. coli BL21(DE3) clones contain plasmid pET22b(+) harboring the gene encoding fusion tags, wild type BSLA or BSLA variants. Each variant contains a single amino acid exchange at a different position chosen randomly and distributed over the entire enzyme sequence of 181 amino acids.