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Table 1 Applied clones and abbreviations

From: Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli

Clone

Abbreviation

E. coli BL21(DE3):pET22b(+)–His6-LOV

His-LOV

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-wt

Wild type enzyme (His-LOV-BSLA)

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Ala1Trp

A1W

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-His10Asp

H10D

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Ile12Cys

I12C

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Phe17Pro

F17P

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Lys23stop

K23stop

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Ser56Pro

S56P

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Asp91Arg

D91R

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Gly93Tyr

G93Y

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Val99Lys

V99K

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Leu102Trp

L102W

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Ser167Pro

S167P

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Lys170Glu

K170E

E. coli BL21(DE3):pET22b(+)–His6-LOV-BSLA-Gly175Phe

G175F

  1. The investigated E. coli BL21(DE3) clones contain plasmid pET22b(+) harboring the gene encoding fusion tags, wild type BSLA or BSLA variants. Each variant contains a single amino acid exchange at a different position chosen randomly and distributed over the entire enzyme sequence of 181 amino acids.