Comparison of 15
BL21(DE3) clones belonging to respiration behavior Type A and Type B cultivated under inducing conditions. Oxygen transfer rate (top), biomass (middle) and product formation (bottom) as function of time for E. coli BL21(DE3) clones cultivated in Wilms-MOPS mineral autoinduction medium (Type A: white background; Type B: grey background). Clones expressing active Bacillus subtilis lipase A (BSLA) are indicated with (+); clones expressing inactive BSLA are indicated with (−). Cultivation conditions: 37°C, 250 mL flasks, filling volume 10 mL, shaking frequency 350 rpm, shaking diameter 50 mm (in RAMOS); 37°C, 48-well Flowerplate, filling volume 1 mL, shaking frequency 1500 rpm, shaking diameter 3 mm (in BioLector).