Figure 3

Reproducibility of main cultivations of 15 E. coli BL21(DE3) clones under inducing conditions expressing fusion tags, wild type BSLA, or different BSLA variants as specified in Table 1 . Oxygen transfer rate (OTR) as function of time obtained from 2–6 independent replicate experiments performed in Wilms-MOPS mineral autoinduction medium containing 0.5 g/L glucose, 2 g/L lactose and 5 g/L glycerol. According to their respiration behavior, the clones can be classified into Type A (white background) and Type B (grey background). The nine clones belonging to respiration behavior Type A reach an OTR of 15–20 mmol/L/h in both OTR peaks, whereas the six clones belonging to respiration behavior Type B increase up to only about 10 mmol/L/h in their first OTR peak and reach 25–40 mmol/L/h in the second OTR peak. Cultivation conditions: 37°C, 250 mL flasks, filling volume 10 mL, shaking frequency 350 rpm, and shaking diameter 50 mm.