Overview of different cultivation steps, cultivation systems, and measured online and offline data. First preculture is performed in complex non-inducing TB medium, second preculture in non-inducing Wilms-MOPS mineral medium. Both precultures are carried out in 250 mL flasks in a RAMOS (Respiration Activity MOnitoring System) device. The main culture is performed in Wilms-MOPS mineral autoinduction medium in three cultivation systems in parallel: RAMOS device (250 mL shake flasks), separate 250 mL shake flasks and 48-well Flowerplate in a BioLector device. The RAMOS device allows an online measurement of the oxygen transfer rate (OTR) of the culture. Separate shake flasks are used for offline analysis. The BioLector device monitors the formation of biomass (scattered light intensity) and product (fluorescence).