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Table 4 Strains, plasmids and oligonucleotides used in this study

From: Role of L-alanine for redox self-sufficient amination of alcohols

Strains

Relevant characteristics

Reference

E. coli DH5α

F thi-1 endA1 hsdr17(r, m) supE44 ΔlacU169

[19]

(ɸ80lacZΔM15) recA1 gyrA96 relA1

E. coli W3110

F λ INV(rrnD – rrnE)1

[19]

E. coli MG1655

F λ ilvG- rfb-50 rph-1

[19]

E. coli YYC202

ΔaceEF pfl1 poxB1 pps4 rpsL zbi::Tn10

[8]

E. coli BW25113

lacI q rrnB T14 lacZ WJ16 hsdR514 araBA-D AH33 rhaBAD LD78

[20]

W3110/pTrc99A-ald-adh-ta Vf

E. coli W3110 harboring pTrc99A-ald-adh-ta with the transaminase of Vibrio fluvialis

[6]

W3110/pTrc99A-ald-adh-ta Cv

E. coli W3110 harboring pTrc99A-ald-adh-ta with the transaminase of Chromobacterium violaceum

This study

MG1655/pTrc99A-ald-adh-ta Cv

E. coli MG1655 harboring pTrc99A-ald-adh-ta with the transaminase of Chromobacterium violaceum

This study

YCC202/pTrc99A-ald-adh-ta Cv

E. coli YCC202 harboring pTrc99A-ald-adh-ta with the transaminase of Chromobacterium violaceum

This study

BW25113/pTrc99A-ald-adh-ta Cv

E. coli BW25113 harboring pTrc99A-ald-adh-ta with the transaminase of Chromobacterium violaceum

This study

JW0855-1/pTrc99A-ald-adh-ta Cv (BW25113ΔpoxB::kan)

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ , ΔpoxB772::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514; harboring the plasmid pTrc99A-ald-adh-ta with the transaminase of Chromobacterium violaceum

[20]/Transformation in this study

JW2293-1/pTrc99A-ald-adh-ta Cv (BW25113ΔackA::kan)

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ , ΔackA778::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514; harboring the plasmid pTrc99A-ald-adh-ta with the transaminase of Chromobacterium violaceum

[20]/Transformation in this study

JW2294-1/pTrc99A-ald-adh-ta Cv (BW25113Δpta::kan)

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ , Δpta779::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514; harboring the plasmid pTrc99A-ald-adh-ta with the transaminase of Chromobacterium violaceum

[20]/Transformation in this study

Plasmids

Relevant characteristics

Reference

pTrc99A-ta Vf

pTrc99A carrying ta of Vibrio fluvialis

This study

pTrc99A-ta Cv

pTrc99A carrying ta of Chromobacterium fluvialis

This study

pTrc99A-ald-adh-ta Vf

pTrc99A carrying ald-adh-ta Vf synthetic operon

[6]

ald from B. subtilis 168

adh from B. stearothermophilus

ta from V. fluvialis

pTrc99A-ald-adh-ta Vf_mut

pTrc99A-ald-adh-ta Vf with BamHI cut site upstream of ta Vf

This study

pTrc99A-ald-adh-ta Cv

pTrc99A carrying ald-adh-ta Cv synthetic operon

This study

ald from B. subtilis 168

adh from B. stearothermophilus

ta from C. violaceum

Oligonucleotides

Sequence 5′→ 3′

Use

taVf_RBS_for

CAGACCATGGAATTCGAGCAGGAAACAGACCATGAACAAACCGCAGAGCTG

pTrc99A-ta Vf

taVf_rev

ATCCCCGGGTACCGAGTTACGCAACTTCCGCGAAAAC

pTrc99A-ta Vf

taCv_KpnIRBS_for

CAAGGTACCCAGGAAACAGACCATGCAGAAACAGCGTACCACC

pTrc99A-ta Cv

taCv_BamHI_rev

GTTGGATCCTTAGGCCAGACCACGTGCTTTC

pTrc99A-ta Cv

pTrc99a-ald-adh-taVf_mut_for

GGAAGATAAATAAGGATCCCAGGAAACAGACCATGAAC

pTrc99A-ald-adh-ta Cv

pTrc99a-ald-adh-taVf_mut_rev

CTGGGATCCTTATTTATCTTCCAGGGTCAGAACAACA

pTrc99A-ald-adh-ta Cv

pTrc99a-ald-adh-taCv _for

CAAGGATCCCAGGAAACAGACCATGCAGAAACAGCGTACCACC

pTrc99A-ald-adh-ta Cv

pTrc99a-ald-adh-taCv _rev

GTTGGATCCTTAGGCCAGACCACGTGCTT

pTrc99A-ald-adh-ta Cv