Figure 4
From: Role of L-alanine for redox self-sufficient amination of alcohols
Role of pyruvate oxidase PoxB for conversion of 1,10-decanediol to 1,10-diaminodecane in redox self-sufficient whole cell amination. Amination of 1,10-decanediol was performed in a resting buffer system with 20 mM L-alanine, 100 mM NH4Cl and 10 mM 1,10-decanediol at 42°C. Strain YYC202 lacks poxB (and others) compared to its isogenic parent MG1655, strain W3110ΔpoxB lacks poxB compared to its isogenic parent W3110 and BW25113ΔpoxB lacks poxB compared to its isogenic parent BW25113. All strains carried pTrc99a-ald-adh-ta Cv.