Figure 3
From: Role of L-alanine for redox self-sufficient amination of alcohols
Comparison of the production rate and product formation for redox self-sufficient amination of 1,10-decanediol at 37 °C , 40 °C and 42 °C using whole cell biocatalysts W3110/pTrc99a- ald - adh - ta Vf and W3110/pTrc99a- ald - adh - ta Cv . The cells were taken for whole cell biotransformation after 15 hours of gene expression in LB + 20 mM Mops at 37°C. In a resting system consisting of 50 mM Hepes pH7, 10 mM 1,10-decanediol, 100 mM L-alanine and 100 mM NH4Cl an optical density at 600 nm of 10 was adjusted.