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Table 5 Posttranslational activation of apo-HRP with hemin

From: Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

Hemin [μM]

Specific activity [U/mg] after 5 min of incubation

Specific activity [U/mg] after 72 h of incubation

-

34.8

35.3

1.0

42.4

67.0

5.0

46.8

75.4

10.0

51.8

77.8

  1. Cell-free cultivation supernatant was incubated with the indicated concentrations of hemin and volumetric HRP activity was measured after 5 min and 72 h.