Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

Table 2 Strain characteristic physiological parameters determined for the benchmark, HEM1 and HEM3 strain

Strain	Δtime _adapt [h]	Y _X/MeOH [C-mol/C-mol]	Y _CO2/MeOH [C-mol/C-mol]	C-balance	q _MeOH [mmol/g/h]	q _p [U/g/h]	r _p [U/L/h]
Benchmark	8.2	0.02	1.00	1.02	0.75	1.11	37.5
HEM1	8.2	0.04	0.94	0.98	0.69	1.10	35.9
HEM3	7.9	0.03	0.93	0.96	0.70	0.49	17.1

Δtime_adapt, time for adaptation of the culture to methanol; Y_X/MeOH, Y_CO2/MeOH, yields of biomass or CO₂ per C-mol of substrate methanol; C-balance, sum of Y_X/MeOH and Y_CO2/MeOH which ideally should result in 1.0; q_MeOH, average specific uptake rate of methanol during consecutive methanol pulses; q_p, specific HRP productivity; r_p, volumetric HRP productivity calculated from the point of induction until the end of cultivation.

ISSN: 1475-2859