Figure 5
From: Expression of the functional recombinant human glycosyltransferase GalNAcT2 in Escherichia coli
SDS-PAGE analysis and specific activity (SA) of HisDapGalNAcT2 compared to rhGalNAcT2 using the acceptor peptide EA2 or Filgrastim™. All reactions were initiated with 0.2 mM UDP-GalNAc and 1 mM EA2 or 3 μg Filgrastim™ per well and proceeded at 37°C for 60 min. (A, B, D, E) Absorbance at 620 nm plotted against glycosyltransferase input in μg. (C, F) Comparison of the SA of HisDapGalNAcT2 and rhGalNAcT2. Denatured (5 min at 95°C) and untreated (10 min at room temperature) protein samples of both glycosyltransferases were separated by either reducing (G) or non-reducing (H) SDS-PAGE. The emerging double band is indicated by an arrow.