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Table 1 E. coli strains and plasmids used in this study

From: Production of cinnamic and p-hydroxycinnamic acid from sugar mixtures with engineered Escherichia coli

Strain Characteristics Reference
W3110 Escherichia coli F, λ, INV(rrnD rrnE)1 ATCC 27325
WPJ W3110 transformed with plasmid pJLBaroG fbr tktA This work
WPJRg W3110 transformed with plasmid pJLBaroG fbr tktA and pTrcPALRg This work
WPJAt W3110 transformed with plasmid pJLBaroG fbr tktA and pTrcPALAt This work
W(pheA)Rg W3110 (pheA::Km) transformed with plasmid pJLBaroG fbr tktA and pTrcPALRg This work
W(pheA)At W3110 (pheA::Km) transformed with plasmid pJLBaroG fbr tktA and pTrcPALAt This work
VH33 Derivative of W3110 (ΔptsH, ptsI, crr::Km, ΔlacI, lacZ::loxP, PgalP::Ptrc) [23]
VPJ VH33 transformed with plasmid pJLBaroG fbr tktA This work
VPJRg VH33 transformed with plasmid pJLBaroG fbr tktA and pTrcPALRg This work
VPJAt VH33 transformed with plasmid pJLBaroG fbr tktA and pTrcPALAt This work
Plasmids
pJLBaroG fbr tktA aroG fbr under control of the lacUV5 promoter; and tktA under its native promoter, carries lacIq and tet genes [26]
pTrc99A Cloning vector, carries bla and lacIq genes and trc promoter [27]
pTrcPALRg PAL gene from R. glutinis gene cloned in pTrc99A This work
pTrcPALAt PAL gene from A. thaliana cloned in pTrc99A This work