Table 2 Primers used in this work
From: Metabolic engineering of the fungal D-galacturonate pathway for L-ascorbic acid production
Primer | Sequence | Description |
|---|---|---|
P1 | AGGCATCTGTCTGAGAGGCAACCGTGGCGA | Amplification of gaaA/C promoter, forward |
GAGTCCGCATTCTTTGATCTGCTGTTAGTT | ||
P2 | GGTGACGAAGTGTGCGATTGAGCGTGATAA | Amplification of gaaA/C promoter, reverse |
AACGAAACATTGTGATTGCTGTGGTGTAAA | ||
P3 | TAAGTTGGAGAAGTTGTTTCCGTCGCTCGAT | Amplification of gaaA terminator, forward |
GCCATTTGAATACCTTAGAGAAGCTTGTATG | ||
P4 | CGTCTCTCCGCATGCCAGAAAGAGTCACCGG | Amplification of gaaA terminator, reverse |
TCACTGTACCATCTCCATCTCCTTCCCG | ||
P5 | GCCCCCCCTCGAGGTCGACGGTATCGATAAGC | Amplification of gaaC terminator, forward |
TTGATATCGAATTCCTGTTGGAGAGAGGGTGTGT | ||
P6 | CCCAGCCCCAGGTCCGCCACCCGCAGAGTTCCGT | Amplification of gaaC terminator, reverse |
TTGTGATCCATTGTATCATATAGATTATGAC | ||
P7 | ATGCGGACTCTCGCCACG | Amplification of Eg ALase, forward |
P8 | TCACAAACGGAACTCTGCGG | Amplification of Eg ALase, reverse |
P9 | ATGTTTCGTTTTATCACGCT | Amplification of Mg GALDH, forward |
P10 | TCAAATGGCATCGAGCGAC | Amplification of Mg GALDH, reverse |
P11 | GTACAGTGACCGGTGACTCT | Amplification of hph, forward |
P12 | GCTGGAGCTCCACCGCGGTGGCGGCCGCTCTAGA | Amplification of hph, reverse |
ACTAGTGGATCCTTGGAGATTTCAGTAACGTT | ||
P13 | GAGGTCGACGGTATCGATAAGC | Sequencing of the bidirectional Eg/Mg cassette |
P14 | TGATACAATGGATCACAAACGG | " |
P15 | CAACAGAGAACAGACCGCCA | " |
P16 | GTGTTGCGAAGCTGTAGTTGG | " |
P17 | ATTTACACCACAGCAATCAC | " |
P18 | AAAGAAGCGTGTTCGAGTCC | " |
P19 | ATACGGAGGATGAAGCCCTC | " |
P20 | GCCAGCGGAAGGAGATTACG | " |
P21 | GGCAGTGATTGAGGCTGTGG | " |
P22 | AGTAAGCGAAGGAGAATGTG | " |
P23 | AGTACTTTGCTACATCCATACTCC | " |
P24 | ATTCGGACCGCAAGGAATCG | " |
P25 | TGTCGGGCGTACACAAATCG | " |
P26 | AGCCGTGTTTCAATGTCGTG | " |
P27 | CGCTCTAGAACTAGTGGATC | " |
P28 | CAACATTGTCATGTCTGGTGG | qPCR of actin, forward |
P29 | GGAGGAGCAATGATCTTGAC | qPCR of actin, reverse |
P30 | AGGACACGATTACTCTACTTG | qPCR of gaaA, forward |
P31 | GAGCCCATATAATGGAAGTAC | qPCR of gaaA, reverse |
P32 | TCCGGGTGGACCCCGCTAAG | qPCR of EgALase, forward |
P33 | TGAAACACGGCTCCGGCGTC | qPCR of EgALase, reverse |
P34 | TAGCAAGTGGCGCGGTGTCC | qPCR of MgGALDH, forward |
P35 | TCGTGATCTCACCGCCCCGA | qPCR of MgGALDH, reverse |