Table 1 Plasmids and A. niger strains used in this work
From: Metabolic engineering of the fungal D-galacturonate pathway for L-ascorbic acid production
Plasmid | Genes expressed | Promoter |
|---|---|---|
JKp1-EgALase | The codon optimized Euglina gracilis Alase (EgALase) [GenBank: AB306917] | gpdA |
JKp1-Smp30 | The codon optimized rat Smp30 [GenBank: CAA48786] | gpdA |
JKp1-MgGALDH | The codon optimized Malpighia glabra GALDH (MgGALDH) [GenBank: ACG75919] ORF | gpdA |
Bidir-EgALase-MgGALDH | EgALase and MgGALDH | gaaA/C |
Strain | Genes expressed | Description |
∆gaaB | none | ATCC 1015 with the deleted L-GalA dehydratase gene gaaB |
∆gaaB-Mg | MgGALDH | ∆gaaB with MgGALDH expressed under gpdA promoter |
∆gaaB-Eg-Mg (PgpdA) | EgALase and MgGALDH | ∆gaaB with EgALase and MgGALDH, both expressed under gpdA promoter |
∆gaaB-Smp30-Mg | Smp30 and MgGALDH | ∆gaaB with Smp30 and MgGALDH, both expressed under gpdA promoter |
∆gaaB-Eg-Mg (PgaaA/C) | EgALase and MgGALDH | ∆gaaB with EgALase and MgGALDH, both expressed under bidirectional gaaA/C promoter |