Table 3 Cell-size distribution at different time points of the fed-batch cultivation. Single and budding cells were categorized with a size of < 15 μm, whereas cell clusters were classified to be > 15 μm
Cultivation | Size distribution after batch [%] | Size distribution after non-induced fed-batch [%] | Size distribution after induced fed-batch [%] | |||
|---|---|---|---|---|---|---|
<15 μm | >15 μm | < 15 μm | > 15 μm | < 15 μm | > 15 μm | |
DoE1 | 53.0 | 47.0 | 41.5 | 58.5 | 79.8 | 20.2 |
DoE2 | 56.8 | 43.2 | 64.8 | 35.2 | 79.9 | 20.1 |
DoE3 | 54.7 | 45.3 | 60.5 | 39.5 | 64.7 | 35.3 |
DoE4 | 38.3 | 61.7 | 57.1 | 42.9 | 80.6 | 19.4 |
DoE5 | 53.8 | 46.2 | 74.8 | 25.2 | 73.3 | 26.7 |
DoE6 | 55.7 | 44.3 | 70.9 | 29.1 | 81.0 | 19.0 |
DoE7 | 36.3 | 63.7 | 42.5 | 57.5 | 75.8 | 24.2 |
DoE8 | 40.8 | 59.2 | 47.0 | 53.0 | 71.0 | 29.0 |
DoE9 | 49.9 | 50.1 | 69.5 | 30.5 | 90.4 | 9.6 |
DoE10 | 48.2 | 51.8 | 69.4 | 30.6 | 89.1 | 10.9 |
average | 48.8 ± 7.6 | 51.3 ± 7.6 | 59.8 ± 12.3 | 40.2 ± 12.3 | 78.6 ± 7.8 | 21.4 ± 7.8 |
wildtype OCH1 strain | 87.5 | 12.5 | 87.3 | 12.7 | 86.5 | 13.5 |