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Table 2 Strain characteristic parameters of the recombinant P. pastoris Δ och1 strain induced under different conditions

From: Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

Cultivation

q s Gly batch

q s MeOH adapt

q s MeOH fed-batch

MeOH accum

Y CO2/S

Y X/S

C-balance

[mmol∙g −1 ∙h −1 ]

[mmol∙g −1 ∙h −1 ]

[mmol∙g −1 ∙h −1 ]

[g∙L −1 ]

[C-mol∙C-mol −1 ]

[C-mol∙C-mol −1 ]

DoE1

1.42

0.23

0.20

-

0.80

0.21

1.01

DoE2

1.40

0.26

0.26

-

0.80

0.20

1.00

DoE3

1.38

0.08

0.18

0.68

0.82

0.23

1.05

DoE4

1.28

0.09

0.16

4.90

0.82

0.24

1.06

DoE5

1.26

0.16

0.21

-

0.83

0.26

1.09

DoE6

1.25

0.17

0.20

-

0.75

0.32

1.07

DoE7

1.39

0.16

0.20

-

0.71

0.29

1.00

DoE8

1.40

0.18

0.24

-

0.69

0.33

1.02

DoE9

1.42

0.15

0.20

-

0.75

0.28

1.03

DoE10

1.42

0.16

0.21

0.20

0.80

0.27

1.07