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Table 1 Experimental plan for the multivariate analysis of the 3 factors temperature, pH and dO 2 and their effects on different response parameters

From: Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

Cultivation

Run order

Temperature [°C]

pH

dO 2 [%]

DoE1

9

20

5

30

DoE2

1

20

5

10

DoE3

8

30

5

30

DoE4

2

30

7

30

DoE5

6

30

7

10

DoE6

3

25

6

20

DoE7

10

20

7

10

DoE8

4

20

7

30

DoE9

7

25

6

20

DoE10

5

30

5

10