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Figure 1 | Microbial Cell Factories

Figure 1

From: Quality assessment and optimization of purified protein samples: why and how?

Figure 1

Experimental protein quality control methodological work-flow. A) The properties (purity & integrity, homogeneity, activity) to be assessed for each new protein sample are listed on the upper left. First-line methods are essential and should be used systematically for a full quality control assessment. Complementary methods can be added depending on the protein sample peculiarities and quality control requirements. Similarly, methods for sample optimization monitoring are grouped below in two categories: first-line and complementary. B) The work flow has to be followed step-by-step starting with the “protein production and purification” green box. For each step, achievement of quality criteria is indicated by a green arrow (passed) while failure is indicated by a red arrow (failed). In case of failure, process optimization has to be carried out as indicated by black arrows. Initial sample assessment is sufficient if a sample is only produced once and used directly without storage (orange arrow at the bottom left). In contrast, if samples have to be stored for an undetermined period of time and produced several times, the sample optimization part of the work-flow should be performed thoroughly. If no appropriate storage conditions can be found, one should work only with fresh preparations (orange arrow on the right).

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