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Figure 2 | Microbial Cell Factories

Figure 2

From: An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains

Figure 2

Different inducible promoters compared to the constitutive aidA promoter for surface expression of recombinant protein. A. Representative dot plot from flow-cytometric analysis of E. coli BL21 (paidA-ZIgG-EC). Fluorescence intensity corresponding to IgG-binding on the y-axis and fluorescence intensity corresponding to surface expression level (HSA-binding) on the x-axis. B. The different inducible promoters AraBAD, RhaBAD and T7 before the expression cassette (EC). C. Representative dot plot from flow-cytometric analysis of E. coli BL21 (pRhaBAD-ZIgG-EC). D. Representative dot plot from flow-cytometric analysis of E. coli BL21 (pAraBAD-ZIgG-EC).

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Microbial Cell Factories

ISSN: 1475-2859

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