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Table 1 Yield of biomass, SUMO-SA2 and SA2 (mg) produced per liter of LB or ZYM medium

From: Optimization of the recombinant production and purification of a self-assembling peptide in Escherichia coli

Medium

Wet Biomass (g)

SUMO-SA2 (mg)

Expected amount of SA2 (mg)

Amount of cleaved SA2 (mg)

Amount of purified SA2 (mg)

LB + 1 mM IPTG

2.18 ± 0.11

63 ± 2

5.04 ± 0.18

4.26 ± 0.15

3.20 ± 0.20

ZYM (Auto induction)

7.62 ± 0.03

243 ± 5

19.44 ± 0.45

16.21 ± 0.32

12.16 ± 0.43

  1. These results show that compared to induction with IPTG, the auto induction medium significantly increased SUMO-SA2 production and subsequently the yield of purified SA2 peptides (>3.8 times).