Figure 6From: Optimization of the recombinant production and purification of a self-assembling peptide in Escherichia coli Schematic representation of IMAC purification and the peptide loss in 2 nd IMAC purification after cleavage of SUMO-SA2 by SUMO protease. Released peptides are able to form aggregates with cleaved or uncleaved SUMO-SA2. Collected peptide in the flow through is low and most peptides stick to the column through the His tag of bigger proteins.Back to article page