Use of non-growing Lactococcus lactis cell suspensions for production of volatile metabolites with direct relevance for flavour formation during dairy fermentations

Table 1 Development of incubation mixture for volatile production by non-growing cells

	3-methylbutanal	Fold change	3-methylbutanol	Fold change
leucine + cells	1.0E + 08	1.0	2.2E + 07	1.0
leucine + glucose	6.4E + 06	0.1	3.8E + 06	0.2
leucine + glucose + cells	2.5E + 08	2.4	5.7E + 07	2.6
leucine + α-ketoglutarate + cells	3.6E + 08	3.6	7.7E + 08	35.2
leucine + α-ketoglutarate + pyrydoxal-5-phosphate + cells	6.2E + 08	6.2	5.9E + 08	27.1

Fold change is displayed against the incubation with leucine and cells.
Effect of α-ketoglutarate and pyridoxal phosphate on 3-methyl butanal and 3-methyl butanol production (GCMS-peak area) by L.lactis 1157 in a buffer system containing 100 mM phosphate, pH 6,0, 2 mM NADH and 10 mM leucine.

ISSN: 1475-2859