Table 5 Primer sequence used in this study
Primer name | Primer sequence (5’→3’) |
|---|---|
serAF | TCTAGAGTCGAGCACAGCCGTATTCTAAGGAGGTCAAATGTGAGCCAGAATGGCCGTCC |
serAR | GTAGTTTTGTCTCCGTTTAATTAGTCTTTAAGATCTTTAGCGAGCCAGATCCATCCACA |
serBF | CGCTAAAGATCTTAAAGACTAATTAAACGGAGACAAAACTACATGCCTAACATTACCTG |
serBR | CCTCCTTTATAAAATAGATTACTACCTTGGATCCTTACTTCTGATTCAGGCTGCCTGAG |
serCF | GTAAGGATCCAAGGTAGTAATCTATTTTATAAAGGAGGAGGTAATGGCTCAAATCTTCA |
serCR | CTTGCATGCCTGCAGGTCGACTTAACCGTGACGGCGTTCG |
pTrc101R | TCTAGAGGATCCCCGGGTACCGAGCTC |
pTrc101F | TCATGGTTGAGTTCGAACGCCGTCACGGTTAAGTCGACCTGCAGGCATGCAA |
pgk_F | TCGAGCTCCGCACCACAAAAACGACACCATAGGGGGGCAAACGATGTCTGTAATTAAGA |
pgk_R | GTGCGGAGCTCTTACTTCTTAGCGCGCTCTT |
Primers for genetic manipulation | |
SdaAp U_F | TATACCGCCTTCCGCCGTTG |
SdaAp U_R | AATGAGCCGGATGATTAATTGTCAACAGCTCATTAGTCAGGGTTTCACACCAA |
SdaAp T_F | ATGAGCTGTTGACAATTAATCATCCGGCTCATTACCCTGTTATCCCTACTAAGC |
SdaAp T_R | TACTCCGTCGACTGTGTGACCACACATTATACGAGCCGGATGATTAATTGTCAACAGCTCATTAGGGATAACAGGGTAATGTACC |
SdaAp L_F | ATAATGTGTGGTCACACAGTCGACGGAGTAACGACTCTCGTAAATAAGGAGTTTAAAGTGATTAGTCTATTCGACAT |
SdaAp L_R | AAACCGGGAATACTGTCGAT |
Tet U | AGCTGAGTCAGGAGATGCGG |
Tet R | AGCTGTTTCCTGGTTTAAAC |
Ser U_F | CTGGTCGAAACTCAATAACTCC |
Ser U_R | TTAATTGTCAACAGCTCATCCGCATCTCCTGACTCAGCTTTCCCCTCACCACGTTGCGT |
Ser L_F | GTACAGTACTTCAATTTGTTTAAACCAGGAAACAGCTATGTCTGTAATTAAGATGACCG |
Ser L_R | AGCATTATCAGAGAGTTGCCAT |
PaceEF U_F | GCCAGAACTTCGAATTGCTC |
PaceEF U_R | TGTCAACAGCTCATCCGCATCTCCTGACTCAGCTGGGTTATTCCTTATCTATC |
PaceEF L_F | GTACAGTACTTCAATTTGTTTAAACCAGGAAACAGCTATGTCAGAACGTTTCCCAA |
PaceEF L_R | CCAGTTCCAGATTACCCGGAT |
Pzwf U_F | TCCGCACTGAAAGAAATCGAA |
Pzwf U_R | TGTCAACAGCTCATCCGCATCTCCTGACTCAGCTCGCATTCTCCTTAAGTTAAC |
Pzwf L_F | GTACAGTACTTCAATTTGTTTAAACCAGGAAACAGCTATGGCGGTAACGCAAACAG |
Pzwf L_R | ATTCAGTTTTGCCTCGCCAAG |
Pedd U_F | CAGAAACCTTTAATCAGACGCATC |
Pedd U_R | ACAGGGTAATCTAGGACTGAGCTAGCTGTCAACCATAAAGGATAAGCGCAGAT |
Pedd T_F | CTTTATGGTTGACAGCTAGCTCAGTCCTAGATTACCCTGTTATCCCTACTAAGCACT |
Pedd T_R | TATACCTAGGACTGAGCTAGCTGTCAACCATAAAGAAGGGATAACAGGGTAATGTACCA |
Pedd L_F | ACAGCTAGCTCAGTCCTAGGTATAATGCTAGCACGAACAGGCGTTTCAGTCATAAATCC |
Pedd L_R | CGCAACATGCTTTTCAAAGAGG |
Primers for RT-PCR | |
RTsdaA_F/R | CTATGAAGGCAGGTAAACAG/CGAGTAACGCTATCCAGTA |
RTpgk_F/R | GTTCTAAAGTATCTACCAAACTG/ATACCACCACCAACAATC |
RTSerA_F/R | CGATGGTGAG TGGAAACGCT/CGTAGGCAAGGATCTCATCA |
RTSerB_F/R | GAGATCATGGACGGTAAA/ CAGAGTTTTCGCTTTGTA |
RTSerC_F/R | TATTCCATCCTCAACGATA/GACCAGACCAGATAGATA |
RTzwf_F/R | TTGCTAACTCCCTGTTTGT/CTTCTTCTGCCACGGTAA |
RTaceE_F/R | TTACGAAGTTGCTGTCAT/AGCGTAGTGATGTAGTAGTA |
RTaceF_F/R | AAATCCTGGTGAAAGTTG/TTCCATAGAAGCCTTGTC |
RTlpd_F/R | CAGCAAGAAATTCAACCT/CCTTCCATCGTCACATAA |
RTrrsA_F/R | TACGACCAGGGCTACACACG/ATCCGGACTACGACGCACTT |