Table 4 Strains and plasmids used in this study

Strains

E. coli DH5α

Coli Genetic Stock Center strain (CGSC) No. 12384

CGSC^a

E. coli JM109

recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac proAB)/F’ [traD36, proAB ⁺, lac ^q lacZΔM15]

TaKaRa (Dalian, China)

SD01

SD01, P _sdaA ::Ptrc

This study

SD02

SD02, SerC:: Ptrc-pgk-SerABC

This study

SD03

SD02, P _edd ::P _J23119

This study

SD04

SD02, P _edd-eda ::P _J23119 , P _zwf ::P_Trc-162

SD06

SD02, P _aceE ::P_Trc-162

This study

SD07

SD04, P _aceE ::P_Trc-162

This study

Tet-Trc-162

JM109, tetA-trc-M1-162-glyA

Lab collection

Plasmids

pTrc101

Expression vector, pSC101 replication, constitutive trc promoter, Cm^r

This study

pTSer

pSC101 replication, Cm^r, Ptrc -serA-serB-serC

This study

pTPSer

pSC101 replication, Cm^r, P _trc - pgk-serA-serB-serC

This study

pTPgk

pSC101 replication, Cm^r, P_trc-pgk

This study

pBHR68

pBluscript SK(-) derivative, phbA _Re , phbB _Re , phbC _Re cloned from R. eutropha

[34]

pTKRED

pSC101 replication, temperature sensitive replication origin, Spc ^r, P _araBAD -driven I-SceI gene, Red recombinase expression plasmid, lac-inducible expression

[35]

pTKS/CS

p15A replication, Cm ^r, Tet ^r, I-SceI restriction sites

[35]