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Table 2 Comparison of the intracellular NADP + , NADPH concentrations and [NADPH]/[NADP+] ratios of the recombinant strains without the PHB synthesis genes

From: Engineering of Serine-Deamination pathway, Entner-Doudoroff pathway and pyruvate dehydrogenase complex to improve poly(3-hydroxybutyrate) production in Escherichia coli

Strains a

NADP + (μmol/gCDW) b,c

NADPH (μmol/gCDW) c

[NADPH]/[NADP + ] ratio c

JM109

0.99±0.02

0.72±0.02

0.73±0.02

SD02

1.00±0.02

0.73±0.01

0.73±0.01

SD04

0.91±0.04

0.84±0.03

0.92±0.01

SD07

0.86±0.02

0.77±0.03

0.90±0.01

  1. aCells were cultivated in MS medium containing 10 g/L of glucose at 37°C for 6 h.
  2. bCDW The average cell dry weight for all of the strains was 0.38 g/liter per optical-density (OD600) unit of culture.
  3. cThe data shown are the average and standard deviations of three parallel experiments.
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Microbial Cell Factories

ISSN: 1475-2859

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