Skip to main content

Table 1 Cell growth, PHB production, and by-products formation by strains study at the end of the cultivation

From: Engineering of Serine-Deamination pathway, Entner-Doudoroff pathway and pyruvate dehydrogenase complex to improve poly(3-hydroxybutyrate) production in Escherichia coli

Strains a

CDW (g/L) c

PHB (g/L) c

PHB content (%CDW) c

Pyruvate (g/L) c

Acetate (g/L) c

Specific glucose uptake rate (g/g rCDW·h) b, c

JM109 (pBHR68)

3.85±0.09

2.08±0.03

54.0±0.57

ND

0.18±0.01

0.38±0.01

SD02 (pBHR68)

6.30±0.11

4.86±0.09

77.2±0.32

ND

ND

0.64±0.01

SD03 (pBHR68)

4.29±0.04

2.90±0.10

67.6±1.98

0.34±0.01

0.84±0.04

0.71±0.02

SD04 (pBHR68)

5.05±0.24

3.39±0.17

67.0±1.33

0.51±0.03

0.33±0.03

0.73±0.01

SD06 (pBHR68)

6.70±0.14

4.63±0.12

69.1±0.50

ND

ND

0.50±0.01

SD07 (pBHR68)

6.83±0.19

5.54±0.15

81.1±1.20

ND

ND

0.93±0.01

  1. astrains were cultured for 48 h in mineral salt medium supplemented with 1 g/L yeast exact and 20 g/L glucose at 37°C at a rotation rate of 220 rpm under aerobic conditions;
  2. bThe specific glucose uptake rate was determined between 0 h and 24 h of the fermentation. rCDW: residual cell dry weight.
  3. cData were expressed as average values and standard deviations (SD) of three parallel studies.
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us