Figure 5
From: De novo production of the monoterpenoid geranic acid by metabolically engineered Pseudomonas putida
Production of geranic acid by P. putida DSM 12264 expressing GES and MVA in a fed-batch bioprocess. Indicated are the OD600 value (blue) and the concentration of de novo produced geranic acid (red) over time using P. putida DSM 12264 expressing GES and MVA in the bioreactor. Cells were grown in a bioreactor in E2 medium (containing 10% LB and 30 mg/L kanamycin, 4.6 g/L glycerol) for 24 h before induction of gene expression with 0.2% (w/v) L-rhamnose (t = 0). Geranic acid concentration was determined via HPLC over time. Mean values of two independent experiments, indicated by error bars, are given.