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Figure 3 | Microbial Cell Factories

Figure 3

From: De novo production of the monoterpenoid geranic acid by metabolically engineered Pseudomonas putida

Figure 3

Production of geraniol and geranic acid by P. putida DSM 12264 expressing GES. Indicated are the concentrations of de novo produced geraniol (blue) and geranic acid (red) over time using P. putida DSM 12264 expressing GES. Cells were grown in shake flasks in E2 medium (containing 10% LB and 30 mg/L kanamycin, 4.6 g/L glycerol) at 30°C and GES expression was induced 3 h after inoculation with 0.2% (w/v) L-rhamnose. Triangles indicate P. putida DSM 12264 with empty plasmid, circles indicate P. putida DSM 12264 pMiS1-ges. Geraniol and geranic acid concentrations were determined via HPLC over time. Mean values of two independent experiments, indicated by error bars, are given. For detailed statistical analysis, please see Additional file 3.

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