Figure 1
From: An agmatine-inducible system for the expression of recombinant proteins in Enterococcus faecalis
Genetic organization of the AGDI cluster of E. faecalis V583 and putrescine production. A. The AGDI cluster of E. faecalis is composed of five genes, aguR encoding a putative regulator, followed by aguB, aguD, aguA and aguC which encode the putrescine biosynthesis pathway (accession number NC_004668). The P aguR and P aguB promoters are shaded and their predicted transcripts are indicated below (dotted arrows). B. Putrescine production by E. faecalis V583 in 24 h cultures grown with 0, 0.05, 0.1, 0.25, 0.5, 1, 5, 10 or 20 mM agmatine. Values are the means of three independent assays and error bars denote standard deviation.