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Figure 1 | Microbial Cell Factories

Figure 1

From: High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases production of human interleukin-10

Figure 1

High-temperature cultivation of an rhIL-10 expression strain increases cell death. (A) Schematic representation of the rhIL-10 expression cascade. (B) The rhIL-10 expression strain (clone `H') was methanol-induced at either 20°C or 30°C in a 10-L fermentor. The production of rhIL-10 was measured by an ELISA assay. (C) Yeast cell viability was examined by PI staining and analyzed by flow cytometry. The statistical results are presented as the mean ± SD. (**P <0.01, ***P <0.001).

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Microbial Cell Factories

ISSN: 1475-2859

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