Figure 5
Display of antigenic fragments from C. trachomatis and the influenza A virus by attenuated Salmonella . (A) S. Typhimurium SL3261 cells expressing HbpD(Δd1) or HbpD-MOMPIV-MOMPII. Cells were treated with Proteinase K (+ pk) or mock-treated (Δ pk) before analysis by Coomassie stained SDS-PAGE. (B) Cells from A were analyzed by immunoblotting using antibodies against the Hbp β-domain, chlamydial MOMP or OmpA as indicated. (C) S. Typhimurium SL3261 cells expressing HbpD(Δd1) or HbpD-HA2stem-M2e-NP/PA/M1. Cells were as described under A. (D) Cells from C were analyzed by immunoblotting using antibodies against the Hbp β-domain, influenza M2e and OmpA as indicated. Non-cleaved Hbp species (*), proteolytic fragments of the Hbp-derivatives (f) and a truncate of HbpD-HA2stem-M2e-NP/PA/M1 (>) are indicated. Molecular weight markers (kDa) are shown at the left side of the panels.