Figure 2
Secretion of multiple antigens fused to the Hbp autotransporter. (A-B) Hbp constructs were expressed in E. coli MC1061 and the equivalent of 0.03 OD660 units cells (c) and corresponding culture medium (m) samples was analyzed by SDS-PAGE and Coomassie staining. (A) Expression and secretion of Hbp, Hbp-Ag85B[N+C] and Hbp-Ag85B[C+N]. (B) Expression and secretion of Hbp, Hbp-Ag85B[C+N], Hbp-Ag85B[C+N]-ESAT6 and Hbp-Ag85B[C+N]-ESAT6-RV2660c. (C) Samples described under B were analyzed by immunoblotting using the antibodies indicated. Cleaved Hbp passenger (>) and β-domain (β) are indicated. Molecular weight markers (kDa) are shown at the left side of the panels.