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Figure 5 | Microbial Cell Factories

Figure 5

From: The Aspergillus nidulans Zn(II)2Cys6transcription factor AN5673/RhaR mediates L-rhamnose utilization and the production of α-L-rhamnosidases

Figure 5

Expression of rhaA , rhaE and rhaR in a wild type strain in the presence of diverse carbon sources. Total RNA was isolated from the wild type biA1 strain AR5 after 18 h of pregrowth in 0.1% fructose and transfer to inducing (1% L-rhamnose), inducing-repressing (1% L-rhamnose + 1% glucose; R + G), non-inducing-repressing (i.e. 0.1% fructose, 2% lactose and 1% glucose), and starvation (no C source) conditions. Amplifications were reduced to 23 (actA), 24 (rhaA), 30 (rhaE) and 32 (rhaR) cycles in order to obtain semi-quantitative data. The constitutively expressed actA gene was included as a control.

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