Figure 4
Disruption of rhaR leads to reduced induction of catabolic genes and reduced growth on L-rhamnose. (A) Vegetative growth of ΔrhaR mutants on different nutrient sources. Equal numbers (3 × 103/3 μl) of spores of the rhaR + untransformed strain (AR198), the ΔrhaR strains (AR225 and AR227) and the riboB2::AfriboB nutritional controls (AR271 and AR272) were spotted on solid media containing different carbon and nitrogen sources and incubated for 5 days. (B) Expression of lraC under different growth conditions (-, no carbon source; R, rhamnose) and in diverse rhaR genetic backgrounds. Total RNA was reverse transcribed and subjected to amplifications using specific primers for actA (control) and lraC. Amplifications were reduced to 22°Cycles in order to obtain semi-quantitative data.