Table 2 Strains and plasmids used in this study
From: Biosynthesis of β-carotene in engineered E. coli using the MEP and MVA pathways
Name | Relevant characteristics | References |
|---|---|---|
Strains | ||
E.coli BL21(DE3) | F- ompT hsdSB (rB -mB -) gal dcm rne131 λ(DE3) | Invitrogen |
E.coli DH5α | deoR, recA1, endA1, hsdR17(rk-,mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 | Takara |
YJM39 | E.coli BL21(DE3)/pAC-BETA | This study |
YJM40 | E.coli BL21(DE3)/pYJM40, pAC-BETA | This study |
YJM41 | E.coli BL21(DE3)/pYJM41, pAC-BETA | This study |
YJM42 | E.coli BL21(DE3)/pYJM42, pAC-BETA | This study |
YJM43 | E.coli BL21(DE3)/pYJM43, pAC-BETA | This study |
YJM44 | E.coli BL21(DE3)/pYJM44, pYJM14, pAC-BETA | This study |
YJM45 | E.coli BL21(DE3)/pYJM45, pYJM14, pAC-BETA | This study |
YJM46 | E.coli BL21(DE3)/pYJM46, pYJM14, pAC-BETA | This study |
YJM49 | E.coli BL21(DE3)/pYJM49, pYJM14, pAC-BETA | This study |
Plasmids | ||
pETDuet-1 | pBR322 ori, lacI T7lac, Ampr | Novagen |
pET-28a(+) | pBR322 ori, lacI T7lac, Kanr | Novagen |
pCOLADuet-1 | ColA ori, lacI T7lac, Kanr | Novagen |
pBAD 18 | pBR322 ori, araBAD, Ampr | [34] |
pTrcHis2B | pBR322 origin, Ampr | Invitrogen |
pYJM14 | pTrcHis2B carrying ERG12, ERG8, ERG19 and IDI1 from Saccharomyces cerevisiae | [39] |
pAC-BETA | pACYC184 carrying crtE, crtB, crtI and crtY from Erwinia herbicola | [40] |
pYJM40 | pETDuet-1 carrying dxs from Bacillus subtilis | This study |
pYJM41 | pETDuet-1 carrying dxs and fni from Bacillus subtilis | This study |
pYJM42 | pETDuet-1 carrying dxs and fni from Bacillus subtilis, ispA from E.coli BL21(DE3) | This study |
pYJM43 | pETDuet-1 carrying dxs and fni from Bacillus subtilis, GPPS2 from Abies grandis | This study |
pYJM44 | pET-28a(+) carrying mvaE and mvaS from Enterococcus faecalis | This study |
pYJM45 | pCOLADuet-1 carrying mvaE and mvaS from Enterococcus faecalis | This study |
pYJM46 | pBAD18 carrying mvaE and mvaS from Enterococcus faecalis | This study |
pYJM47 | pCOLADuet-1 carrying mvaE and mvaS from Enterococcus faecalis, fni from Bacillus subtilis | This study |
pYJM48 | pCOLADuet-1 carrying mvaE and mvaS from Enterococcus faecalis, fni from Bacillus subtilis, GPPS2 from Abies grandis | This study |
pYJM49 | pCOLADuet-1 carrying mvaE and mvaS from Enterococcus faecalis, dxs and fni from Bacillus subtilis, GPPS2 from Abies grandis | This study |